The Plasmodium falciparum circumsporozoite protein produced in Lactococcus lactis is pure and stable
نویسندگان
چکیده
منابع مشابه
Monoclonal Antibodies against Plasmodium falciparum Circumsporozoite Protein
Malaria is a mosquito-borne infectious disease caused by the parasite Plasmodium spp. Malaria continues to have a devastating impact on human health. Sporozoites are the infective forms of the parasite inside mosquito salivary glands. Circumsporozoite protein (CSP) is a major and immunodominant protective antigen on the surface of Plasmodium sporozoites. Here, we report a generation of specific...
متن کاملConstruct design, production, and characterization of Plasmodium falciparum 48/45 R0.6C subunit protein produced in Lactococcus lactis as candidate vaccine
BACKGROUND The sexual stages of Plasmodium falciparum are responsible for the spread of the parasite in malaria endemic areas. The cysteine-rich Pfs48/45 protein, exposed on the surface of sexual stages, is one of the most advanced antigens for inclusion into a vaccine that will block transmission. However, clinical Pfs48/45 sub-unit vaccine development has been hampered by the inability to pro...
متن کاملgenetic diversity in the circumsporozoite protein gene of plasmodium falciparum from major endemic regions of iran
circumsporozoite protein (csp) is one of the stage specific antigens, which is used for the development of vaccines and to investigate the genetic diversity in plasmodium falciparum malaria parasite. polymerase chain reaction was used for typing of csp genes on 67 positive falciparum malaria patients from sistan and baluchistan province of iran. three fragments were detected for csp gene. twent...
متن کاملDiversity of T Cell Epitopes in Plasmodium falciparum Circumsporozoite Protein Likely Due to Protein-Protein Interactions
Circumsporozoite protein (CS) is a leading vaccine antigen for falciparum malaria, but is highly polymorphic in natural parasite populations. The factors driving this diversity are unclear, but non-random assortment of the T cell epitopes TH2 and TH3 has been observed in a Kenyan parasite population. The recent publication of the crystal structure of the variable C terminal region of the protei...
متن کاملThe Plasmodium circumsporozoite protein is proteolytically processed during cell invasion
The circumsporozoite protein (CSP) is the major surface protein of Plasmodium sporozoites, the infective stage of malaria. Although CSP has been extensively studied as a malaria vaccine candidate, little is known about its structure. Here, we show that CSP is proteolytically cleaved by a papain family cysteine protease of parasite origin. Our data suggest that the highly conserved region I, fou...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2020
ISSN: 0021-9258
DOI: 10.1074/jbc.ra119.011268